Coding

Part:BBa_K2619101:Design

Designed by: Haohui Fang   Group: iGEM18_XJTLU-CHINA   (2018-10-07)


CD63-L7Ae--exosomal membrane fusion protein for RNA packaging


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 736
    Illegal XhoI site found at 747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

L7Ae (Archaeoglobus fulgidus) is an RNA binding protein that can specifically bind to C/D box structure on mRNA. L7Ae is linked to the C-terminus of CD63, and mRNA is packaged during the formation of exosome.



Source

Archael nuclear membrane

References